Indotronix Avani Group · 5 hours ago
Molecular Biologist
San Diego, CA
Contract
Onsite
Entry, Mid Level
2+ years expIndotronix Avani Group is seeking a highly motivated Scientist II to contribute to the research and development of monoclonal antibodies in their Antibody Technologies team. The role involves responsibilities such as protein expression and purification, tissue culture, cell-line engineering, and testing antibodies for flow cytometry applications.
ConsultingMachine LearningSoftware
Hiring Manager
Mohammed Rizwan
Responsibilities
Responsible for purification of antibodies and recombinant proteins using AKTA FPLC and manual methods. Must execute and document work using SOPs and must follow strict GMP requirements under ISO 13485 standards. Coordinates preparation and transfer of purified antibody and protein samples to other functional teams
Executes analytical testing of purified antibodies and proteins using SEC, SDS-PAGE, Western blot, MADLS, and qPCR
Responsible for transfection and/or expression of antibodies and recombinant proteins from various mammalian cell lines, including but not limited to CHO and hybridomas as well as E. coli. Specific requirement for hands-on experience with aseptic technique and cell-culture techniques
Responsible for the execution of experiments with varying complexity using advanced molecular biology techniques
Responsible for the execution of experiments with varying complexity using multi-color Flow Cytometry
Analyze data using FCS Express and FlowJo software, report test results including completion of all required documentation and database entries as required by protocols and SOPs
Qualification
Required
A Bachelor's or Master of Science degree in biochemistry, cellular or molecular biology, immunology, or related discipline and a minimum of 2-4 years of laboratory experience required
Protein purification, molecular biology and tissue culture experience required
Proficient in tissue culture techniques; including transfection of cells for protein expression
Proficient with aseptic technique and cell-culture techniques is required
Protein expression using various systems including but not limited to mammalian cells, insect cells, and bacteria
Protein purification with liquid chromatography and quality assurance using various analytical methods
Software: BD FACS Diva, FlowJo, FCS Express, and Microsoft Office Suite
Preferred
Antibody development experience
Familiarity with assay automation
Immunology
Experience with cell line engineering
Hand-on experience with Flow Cytometry and the ability of analyzing Flow data is preferred
Company
Indotronix Avani Group
Indotronix Avani Group is a consultancy that offers machine learning, cloud computing, software development and SaaS services.
1001-5000 employees
Funding
Current Stage
Late StageLeadership Team
Mitch Meller
Chief Operating Officer
Recent News
2023-12-25
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