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Fred Hutch · 5 hours ago

Shared Resources Specialist II - Mouse Development Lab (MoDeL)

Seattle, WA

Full-time

Onsite

Entry, Mid Level

$31.83/hr - $47.73/hr

2+ years exp

Fred Hutchinson Cancer Center is a leading nonprofit organization focused on cancer treatment and research. The Shared Resources Specialist II - MoDeL will support preclinical research involving transgenic and preclinical mouse models, performing various technical procedures and collaborating with researchers to enhance model development.

Hospital & Health Care

No H1B

Responsibilities

Perform basic molecular biology procedures such as designing and validating PCR assays and performing DNA extraction for Sanger sequencing or NGS workflows

Familiarity with CRISPR techniques, including design, execution, and validation of genome editing experiments is required

Experience designing and validating PCR assays for various applications, such as genotyping, cloning, or expression analysis is required

Hands-on experience in animal care, including mouse colony management, breeding strategies, tissue biopsies for genotyping, and injections through intravenous, intraperitoneal and subcutaneous routes is required

Additional research support tasks may include managing surgical mouse models

This position is expected to provide a high level of support for users by establishing and maintaining positive, collaborative working relationships with faculty and external users

Develop advanced protocols or creative solutions and deliver high quality models with an added ability to guide users in model choice

Be capable of assisting or leading in experimental design collaboratively with users

Oversee supply management and preparation

Develop techniques to review and identify new equipment needs

Develop team SOPs, training materials and guidelines for investigator use

May supervise work-study students, interns and lab aids

Present on new or expanded technologies within MoDeL

Mentor junior staff within the MoDeL

Report results to users; accurately document laboratory activities; coordinate and initiate equipment maintenance visits from vendors; assist MoDeL Administrative Manager in performing billing and cost accounting; foster positive relationships with vendors and service engineers who support MoDeL

Assure that all work done is in accordance with an IACUC approved protocol

Assist investigative staff in the creation of IACUC protocols for use in preclinical models

Other duties as assigned include work on weekends, evenings, or holiday shifts as part of a rotation

Qualification

CRISPR techniquesMolecular biology techniquesPreclinical researchPCR assay designAALAS certificationRodent handling skillsBioinformatics toolsSanger sequencingCollaborative team environmentMS Office proficiencyRecord keepingDatabase managementCommunicationOrganizational skills

Required

Bachelor's Degree and 2+ years of experience, OR

Associate Degree and 5+ years of experience, OR

GED/High School Diploma and 7+ years of experience

Familiarity with CRISPR techniques, including design, execution, and validation of genome editing experiments is required

Experience designing and validating PCR assays for various applications, such as genotyping, cloning, or expression analysis is required

Preferred

AALAS certification at the LAT or higher level is recommended, and persons hired for this position should continue to pursue this certification as soon as possible once eligible until achieved

Demonstrated ability to learn and adapt to new systems and techniques

Ability to learn all PCM procedural techniques

Strong understanding of preclinical research using in vivo models

Advanced rodent handling and procedural/ surgical skills

Small animal surgeries using stereomicroscope

Understanding of molecular biology techniques and concepts

Experience with bioinformatics tools including but not limited to databases such as GenBank, Ensembl, and UCSC Genome Browser, Sequence alignment tools (e.g., BLAST, ClustalW) and protein

Translation and basic sequence analysis

Familiarity with CRISPR techniques, including design, execution, and validation of genome editing experiments

Experience in designing and validating PCR assays for various applications, such as genotyping, cloning, or expression analysis

Proficiency in DNA extraction techniques and experience with Sanger sequencing workflows, including sample preparation and data analysis

Experience working in a core lab environment

Excellent communication and organizational skills

Ability to work in a collaborative team-oriented environment

Experience with MS Office suite of applications (Outlook, Word, Excel, Sharepoint, Teams, One Drive, etc)

Record keeping and database management

Capable of undertaking work with minimal supervision and ability to manage multiple projects simultaneously