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Engage Bio · 5 hours ago

Scientist/Sr. Scientist (Protein & Nucleic Acid Purification & Process Development)

San Carlos, CA

Full-time

Onsite

Mid, Senior Level

$100K/yr - $150K/yr

Engage Bio is redefining what’s possible in genetic medicine with their innovative Tethosome platform. They are seeking a highly motivated Scientist/Senior Scientist to support their R&D team in developing and optimizing protein and nucleic acid purification workflows.

BiotechnologyHealth CareTherapeutics

Hiring Manager

Will Olsen

Responsibilities

Lead development and scale-up of a purification process for an extracellularly secreted His-tagged protein expressed in E. coli

Develop robust mRNA and DNA purification processes

Optimize chromatographic methods (IMAC, ion exchange, SEC, etc.) to maximize yield, purity, and reproducibility

Establish clarification and concentration strategies (centrifugation, filtration, TFF) appropriate for scale-up

Troubleshoot process bottlenecks such as resin fouling, aggregation, low recovery, and host-cell impurity carryover

Perform analytical characterization of products using

Develop, document, and maintain detailed protocols and SOPs

Support early CMC and IND-enabling efforts by generating high-quality process development data

Contribute broadly to platform R&D, including molecular biology, cell-based assays, and in vivo studies as needed

Contribute to upstream optimization including strain selection & growth conditions

Qualification

Protein purificationNucleic acid purificationChromatographic methodsBacterial fermentation optimizationMolecular cloningMammalian cell cultureScientific communicationWriting protocolsMentoring

Required

PhD in Biochemistry, Molecular Biology or a related field. Recent graduates are welcome, as are those with relevant postdoctoral or industry experience

You can quickly identify what you are missing to solve these scientific problems

You explore new (and old!) areas of the literature to develop new ideas

You like to learn new methods

You are relentless in sourcing what you need

You are someone who has had a lot of exposure to chromatographic methods (e.g., IMAC / Ni-NTA, FPLC/AKTA systems)

Strong understanding of protein chemistry