Net2Source (N2S) · 7 hours ago
Research Scientist
Cambridge, MA
Contract
Onsite
Mid, Senior Level
$70/hr - $76.97/hr
1+ years expNet2Source Inc. is an award-winning total workforce solutions company recognized for its rapid growth and commitment to addressing the talent gap. They are seeking a hands-on Contract Scientist with expertise in experimental biology to advance iPSC-derived gut models for evaluating combination therapies for inflammatory diseases, working collaboratively with various teams to deliver actionable data packages.
B2BDeliveryHuman ResourcesInformation Technology
Hiring Manager
Kajal Singh, MBA
Responsibilities
Develop, optimize, and execute iPSC-derived gut model systems:
Differentiation of iPSCs into 3D intestinal organoids
Co-cultures with immune cells (e.g., macrophages, dendritic cells, T cells), and 3D organoid model system
Microphysiological platforms to model barrier and tissue microenvironment
Design and run combination therapy studies:
Plan mono- and pairwise/multi-drug matrices; include biologics, small molecules, and pathway-targeted agents
Execute dose–response and time-course experiments; incorporate inflammatory stimuli (e.g., cytokine cocktails, bacterial products) to model disease-relevant contexts
Analyze combination effects for synergy/additivity/antagonism and generate decision-enabling summaries
Apply quantitative, mechanism-informed readouts:
Barrier integrity and function assays (TEER, permeability, wound healing, mucus layer assays)
Inflammatory and cell-state profiling (cytokine/chemokine panels via ELISA/MSD, qPCR/RT-qPCR, flow cytometry, high-content imaging, immunofluorescence)
Transcriptomic profiling (bulk or single cell preferred) for pathway engagement; collaborate with data scientists for analysis
Implement perturbations and model qualification:
Genetic and pharmacological perturbations to dissect mechanisms and predict combination responsiveness
Assess reproducibility, dynamic range, and throughput; adapt assays to plate-based formats (96-/384-well) and support automation readiness
Collaborate cross-functionally:
Partner with immunology, pharmacology, and data science teams to prioritize combinations, refine hypotheses, and align in vitro models with translational endpoints
Present experimental plans and results to internal stakeholders, including senior leadership; maintain rigorous documentation and SOPs
Ensure compliance with safety practices and proper maintenance of cell lines, reagents, and equipment
Qualification
Required
MS with 5+ years or PhD with 1–2+ years of relevant experience in molecular/cellular biology, immunology, pharmacology, or related field
Hands-on experience with human cell culture and at least one of: iPSC maintenance and differentiation into epithelial/endodermal lineages, Intestinal organoids (enteroids) or epithelial monolayers
Proven ability to execute and troubleshoot functional assays; experience running dose–response studies and quantitative assay analysis
Strong documentation, data analysis, and presentation skills; success working in collaborative, cross-functional settings
Excellent communication skills with ability to present clear scientific data and project updates to diverse audiences
Preferred
Direct experience with iPSC-derived intestinal models, including 3D organoids, 2D monolayers, Transwells, and/or gut-on-chip systems
Expertise in barrier function assays (TEER, permeability), imaging-based analyses, and cytokine profiling
Experience with automation, liquid handling, and high-throughput assay development
Background in inflammatory disease biology and prior industry experience in preclinical combination testing or pharmacology
Company
Net2Source (N2S)
Net2Source (N2S) is a Minority owned global workforce solutions company recognized by SIA as the largest and fastest-growing Total Talent Solutions provider with a presence in 34 countries and in-house Glo-Cal (global and local) teams to support our clients.
1001-5000 employees
H1B Sponsorship
Net2Source (N2S) has a track record of offering H1B sponsorships. Please note that this does not
guarantee sponsorship for this specific role. Below presents additional info for your
reference. (Data Powered by US Department of Labor)
Distribution of Different Job Fields Receiving Sponsorship
Represents job field similar to this job
Trends of Total Sponsorships
2025 (3)
2024 (7)
2022 (8)
2021 (10)
2020 (25)
Funding
Current Stage
Late StageLeadership Team
Ashish Garg
Founder & CEO
Company data provided by crunchbase