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Boston Children's Hospital · 7 hours ago

Research Assistant II-Endocrinology

Boston, MA

Full-time

Onsite

Entry Level

$45K/yr - $70K/yr

1+ years exp

Boston Children's Hospital is seeking a Research Assistant II in the Endocrinology department to study various research topics including cell-based treatments for diabetes and obesity. The role involves performing laboratory experiments, data analysis, and contributing to manuscript writing while supporting general lab operations.

Health CareHospitalMedical

Responsibilities

Isolate intestinal crypts from patient biopsies when needed for new organoid lines

Perform general cell culture work, including human intestinal organoids, mouse intestinal organoids, and human intestinal cell lines. Once sufficiently trained, will help with organoid differentiation experiments and infection of organoids with lentivirus for over-expression and CRISPR knock out studies

Perform general laboratory experiments (initially supervised): RNA isolation, PCR, quantitative PCR, western blots, immunofluorescence staining, and flow cytometry

Use web-based design tools to create novel CRISPR and over-expression lentiviral plasmids

Analyze and interpreting experimental results to help determine the need for repeat studies

Organize data into graphs for presentation which will be shown and discussed at weekly lab meetings

Maintain an organized and thorough lab notebook that records protocols, results, and analysis

Perform literature searches to help with the designing of new experiments (especially with using new cell culture materials, ie small molecules or viruses)

Contribute to the writing and editing of new manuscripts

Support general lab operations (ie, assist in purchasing supplies and maintaining stock solutions)

Qualification

Quantitative PCRWestern blot analysisImmunofluorescence stainingFlow cytometryCell cultureRNA isolationData analysisLiterature searchesEffective communicationProblem-solving skills