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Orion Group · 1 day ago

Scientist II Translational Immunology

Cambridge, MA

Contract

Onsite

Mid Level

$47/hr - $50/hr

4+ years exp

Orion Group is a market leading pharmaceutical company offering an exciting contract opportunity for Scientists in Cambridge, MA. The role involves supporting target identification and validation efforts through various profiling methods and functional experiments in collaboration with different teams within immunology discovery.

Human ResourcesRecruitingStaffing Agency

Hiring Manager

Justin Mertel

Responsibilities

Execute RNAseq library preparations and QC generated libraries using Tapestation/Qubit instruments

Prepare libraries for sequencing and operate Illumina sequencers understanding output and troubleshooting where necessary

Perform multiplexed secreted factor measurements using Luminex

Perform functional experiments with primary human immune cells including stimulation and cell culture

Accurately document project and sample information, protocols, and experimental details through the Electronic Lab Notebook (ELN)

Conduct experiments, troubleshoot, and optimize protocols independently and in collaboration with colleagues to deliver high-quality data in a timely fashion

Interpret findings and communicate effectively to present scientific data, concepts, and recommendations to various internal audiences

Interface with computational biologists and data scientists to generate and interpret RNAseq datasets

Qualification

RNAseq workflowsPBMC handlingMolecular biologyLigand-based assaysNGS technologies

Required

Bachelor's Degree or equivalent education with typically 4 -5 years of experience, or Master's Degree or equivalent education with typically two years of experience

Strong background in basic molecular biology and/or genomics with proficiency in molecular biology lab techniques such as DNA/RNA extraction and nucleic acid QC

Experience with RNAseq workflows including library preparation and QC

Experience handling PBMCs, primary cells, and/or tissues is required

Background in biology, immunology, molecular biology, or other related field

Experience with ligand-based assays such as ELISA, MSD or Luminex