Ohalo · 1 day ago
Tissue Culture Lab Technician (2nd Shift)
South San Francisco, CA
Full-time
Onsite
New Grad, Entry Level
$33/hr - $33/hrOhalo Genetics is seeking a motivated Gene Editing Lab Technician to contribute to the advancement of plant variety development. The successful candidate will assist in various lab operations, including protoplast isolation, data recording, and collaboration with other team members to ensure the success of the gene editing laboratory.
AgricultureBiotechnologyGenetics
No H1B
Responsibilities
Perform enzymatic digestion of plant tissues (e.g., leaf, root, or callus) to isolate viable protoplasts
Transfect protoplasts using PEG-mediated transfection or electroporation methods
Culture transfected protoplasts to regenerate callus
Contribute to the continuous improvement and documentation of SOPs for protoplast workflows
Assess protoplast yield, viability, and cell health using fluorescence and confocal microscopy and viability dyes (e.g., FDA staining)
Maintain accurate and detailed electronic lab notebooks (ELN) and batch records
Compile and analyze experimental data using tools such as ImageJ, GraphPad Prism, or Excel
Prepare samples for downstream molecular analyses, including RNA extraction, protein extraction, and genomic DNA isolation
Collaborate with other Technicians, Research Associates, and Scientists on workflows, ensuring that critical processes are completed
Follow safety protocols and institutional guidelines for working with plant tissue and enzymes
Perform all tasks in a manner consistent with and in support of all company policies and practices related to safety and compliance
Performs other duties as assigned
Qualification
Required
Prior experience in a sterile work environment
Ability to accurately follow verbal and written instructions as well as make suggestions to optimize methodologies
Ability to sit for extended periods of time and do repetitive motions
Ability to take a proactive approach to routine tasks
Must be passionate about learning and enthusiastic about plants and hands-on work
Must have a positive attitude and good interpersonal skills
Ability to ask clarifying questions
Ability to work independently and in a team environment with multiple departments
Must be able to lift up to 50 pounds on intermittent basis and possess the ability to sit for extended periods of time
Candidate must prioritize safety
Preferred
Experience working in a lab including sterile technique, micropropagation, media preparation, microscopy, and DNA extraction
Company
Ohalo
To support a growing population efficiently, we need to shrink the land, water and energy footprint of our crops.
Founded in 2019
51-200 employees
Funding
Current Stage
Growth StageTotal Funding
$40M2024-01-25Series Unknown· $40M
Leadership Team
David Friedberg
Chief Executive Officer
Judson Ward
Founder and Chief Technology Officer
Recent News
Google Patent
2025-02-07
Google Patent
2025-02-07
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