Twist Bioscience · 1 day ago
Research Associate II / Senior Research Associate - Antibody Purification Process Development (South San Francisco, CA)
South San Francisco, CA
Full-time
Onsite
Mid, Senior Level
$83K/yr - $135K/yr
2+ years expTwist Bioscience is a biotechnology company focused on DNA synthesis. They are seeking a motivated Research Associate II or Senior Research Associate to support and improve antibody purification workflows, collaborating with various teams to ensure robust and scalable processes.
B2BBiotechnologyChemicalHealth CareLife Science
Responsibilities
Execute and optimize small- and medium-scale antibody purification workflows across multiple formats, including IgGs, Fc variants, and multi-specific antibodies
Perform capture (e.g. Protein A), polishing steps (SEC, IEX, HIC as appropriate), and buffer exchange using spin columns, gravity columns, and chromatography systems
Support the development, refinement, and standardization of downstream purification SOPs for platform use
Evaluate purification performance metrics, including yield, purity, recovery, aggregation, assembly, and throughput
Troubleshoot common downstream challenges such as low recovery, aggregation, proteolysis, mis-assembly, and/or host-cell protein carryover
Collaborate with upstream expression and analytics teams to close the loop between expression output and purification performance
Develop and support scale-down models that inform larger-scale purification strategies
Contribute to high-throughput or semi-automated purification workflows, including plate-based or parallelized formats
Maintain detailed experimental records and communicate results clearly to cross-functional partners
Manage time and experiments effectively to support multiple concurrent projects
Contribute to a collaborative, proactive, and execution-focused lab culture
Move projects forward efficiently / manage time and resources effectively / prioritize tasks in accordance with the company’s goals
Document and communicate results clearly and succinctly
Be helpful / collaborative / proactive
Stay up to date with new developments, companies, and technologies
Qualification
Required
BS. or M.S. in Cell Biology, Biochemistry, Chemical Engineering, or related fields
Research Associate II Requires a BS and 2 + years of professional experience or a Master's degree and 0-2 years of work experience
Sr Research Associate Requires a BS and 5 + years of professional experience or a Master's degree and 3+ years of work experience
Strong hands-on experience in antibody purification, including: Protein A (and similar) capture and elution optimization, Polishing strategies to reduce aggregates and impurities, Buffer exchange and formulation considerations
Practical experience purifying antibodies from mammalian expression systems (CHO and HEK preferred)
Understanding of how antibody sequence, format, and expression conditions impact downstream behavior (e.g. yield, aggregation, stickiness, recovery)
Experience working with multiple antibody formats, including: Standard IgGs, Fc-engineered variants, Bispecific or multi-chain antibodies
Hands-on experience with antibody quality and purity assessment methods, including: SEC-HPLC or aSEC, CE-SDS or SDS-PAGE, Concentration determination (UV, BCA, etc.)
Experience with antibody characterization methods (e.g. BLI, SPR, DSF, DLS)
Experience with chromatography systems (ÄKTA or similar)
Ability to follow, improve, and help standardize workflows—not just execute protocols blindly
Strong attention to experimental detail, reproducibility, and data quality
Comfort working in a fast-paced, multi-project environment
Ability to follow, improve, and help standardize workflows—not just execute protocols blindly
Preferred
Familiarity with automation or liquid-handling systems is a plus
Experience with high-throughput or semi-automated workflows for expression, purification, and screening is a plus
Familiarity with Python or equivalent programming languages is a plus
Benefits
Bonus
Equity
Generous benefits package
Company
Twist Bioscience
Twist Bioscience is a biotechnology company that offers synthetic DNA solutions, gene synthesis, and variant libraries.
501-1000 employees
H1B Sponsorship
Twist Bioscience has a track record of offering H1B sponsorships. Please note that this does not
guarantee sponsorship for this specific role. Below presents additional info for your
reference. (Data Powered by US Department of Labor)
Distribution of Different Job Fields Receiving Sponsorship
Represents job field similar to this job
Trends of Total Sponsorships
2025 (18)
2024 (14)
2023 (22)
2022 (19)
2021 (4)
2020 (6)
Funding
Current Stage
Public CompanyTotal Funding
$553.11MKey Investors
Capital World InvestorsBiomatics Capital PartnersIllumina
2022-02-09Post Ipo Equity· $250M
2020-01-27Post Ipo Equity· $50M
2018-10-30IPO
Leadership Team
Emily Leproust
CEO
Siyuan Chen
Chief Technology Officer
Recent News
2025-12-30
2025-12-24
Company data provided by crunchbase