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University of Pittsburgh · 3 weeks ago

Laboratory Research Technician III

Pittsburgh, PA

Full-time

Onsite

Entry, Mid Level

2+ years exp

The University of Pittsburgh is seeking a Laboratory Research Technician III for the Ophthalmology Department. The role involves performing independent laboratory work, overseeing specimen inventory, and developing documentation for publication, including manuscripts and grant proposals.

Higher Education

No H1B

Responsibilities

Bibliographic research and experimental design to test and validate scientific hypotheses

Design, implementation, and optimization of experimental workflows; troubleshooting technical and biological parameters in multi-day experiments

Method validation and design of comparative experimental conditions

General laboratory organization, including protocol writing, inventory management, purchasing, and safety documentation

Supervision and training of students

Cell culture at small to large scale (tens to hundreds of millions of cells) for quantitative profiling experiments

Cell harvest, lysis, protein extraction, solubilization, fractionation, and protein biochemistry

Cell line maintenance, passaging, cryostorage, harvesting, aliquot preparation, and mycoplasma testing

Plasmid preparation, competent cells, agar plates, and plaque assays

Bacteriophage detection, preparation of bacterial growth media, buffers, and aliquots

Nucleic acid extraction, purification, and quantification, PCR, cDNA synthesis, and RT-PCR

Western blotting, BCA protein assay, calcium measurement assays, Protein extraction

Mouse (Mus musculus) handling

Retina extraction and embedding in OCT compound

Ear punching and tail sampling, organ dissection, and cryostorage

Injections (intracardiac, subcutaneous, intraperitoneal, retro-orbital), anesthesia, euthanasia

Genotyping by PCR

Excel, ImageJ, Prism, SnapGene

NCBI, Ensembl, Primer3

Photoshop, Adobe Illustrator, EndNote

Be able to work at lab bench for hours at a time, ability to lift up to 25lbs

Qualification

Molecular biologyCell cultureExperimental designProtein biochemistryBiochemical assaysLaboratory managementSoftware proficiencySupervisionBibliographic researchDocumentation

Required

Bachelor's degree in Sciences

2 years of lab experience required

Bibliographic research and experimental design to test and validate scientific hypotheses

Design, implementation, and optimization of experimental workflows; troubleshooting technical and biological parameters in multi-day experiments

Method validation and design of comparative experimental conditions

General laboratory organization, including protocol writing, inventory management, purchasing, and safety documentation

Supervision and training of students

Cell culture at small to large scale (tens to hundreds of millions of cells) for quantitative profiling experiments

Cell harvest, lysis, protein extraction, solubilization, fractionation, and protein biochemistry

Cell line maintenance, passaging, cryostorage, harvesting, aliquot preparation, and mycoplasma testing

Plasmid preparation, competent cells, agar plates, and plaque assays

Bacteriophage detection, preparation of bacterial growth media, buffers, and aliquots

Nucleic acid extraction, purification, and quantification, PCR, cDNA synthesis, and RT-PCR

Western blotting, BCA protein assay, calcium measurement assays, Protein extraction

Mouse (Mus musculus) handling

Retina extraction and embedding in OCT compound

Ear punching and tail sampling, organ dissection, and cryostorage

Injections (intracardiac, subcutaneous, intraperitoneal, retro-orbital), anesthesia, euthanasia

Genotyping by PCR

Excel, ImageJ, Prism, SnapGene

NCBI, Ensembl, Primer3

Photoshop, Adobe Illustrator, EndNote

Be able to work at lab bench for hours at a time

Ability to lift up to 25lbs

Preferred

Molecular biology preferred

Cover Letter

Company

University of Pittsburgh

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The University of Pittsburgh is a top-ranked, public institution in Pennsylvania and a member of the Association of American Universities of leading research universities.

Founded in 1787

Pittsburgh, PA, US

10001+ employees