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Fred Hutch · 4 hours ago

Research Technician III

Seattle, WA

Full-time

Onsite

Mid, Senior Level

$80K/yr - $104K/yr

5+ years exp

Fred Hutchinson Cancer Center is an independent, nonprofit organization focused on cancer treatment and research. The Research Technician III role involves designing and implementing viral sequencing and molecular assays, performing various laboratory techniques, and managing data and laboratory records under the supervision of a staff scientist.

Hospital & Health Care

No H1B

Responsibilities

Independently execute documented protocols, maintain a high level of accuracy for all work and keep detailed records for experiments

Troubleshoot and optimize experimental methods, update the team of any deviations

Proactively prepare reagents for experiments and for general lab use

Perform molecular procedures including but not limited to sequencing sample preparation (sanger, NGS, PacBio, SMART-Seq, etc.)

Design and implement assays for gene expression by high density multiplex PCR and/or digital droplet PCR, RT-qPCR, single cell assays such as SMART-seq

Operate and carry out basic maintenance and QC of laboratory equipment as needed

Generate and refine large nucleotide alignments of diverse sequences

Primer and probe design (PCR, qPCR, NGS, etc.)

Optimization and experimental design experience

DNA and RNA extraction from blood and tissue

Experience using Geneious Prime software

Engage in developing novel assays, modifying and refining procedures and techniques to obtain optimal results

Review the literature to stay informed on the HIV/TB/SARS-Cov-2 vaccine field and/or new emerging immunological assays

Work well as team-member, participate in positive communication, and foster an environment that allows all laboratory staff to work well together

Qualification

Molecular biology techniquesNGS library preparationPrimerProbe designData managementImmunology experienceGeneious PrimePCR techniquesMicrosoft OfficeAttention to detailTeamworkCommunication skills

Required

Bachelor's degree in related scientific field or equivalent combination of education and experience

5 years of experience working in a lab outside of school coursework (or equivalent combination of advanced degree and work experience)

Preferred

Experience with immunology, COVID, HIV/AIDS, TB and/or molecular assay including HIV viral sequencing including primer design, NGS library preparation (Illumina, PacBio, Smart-Seq), Real time or digital droplet PCR are strongly desired including experience in primer and probe design

Motivation and adaptability are essential, as is a keen interest in the HIV vaccine field

Great attention to detail, well-organized, self-motivated, and able to efficiently manage time and experiments

Willing to work with biohazardous/infectious agents (e.g., bloodborne pathogens, including SARS-CoV-2 & HIV-1) also be flexible and willing to work some evenings and weekends

Demonstrated scientific knowledge in the area of study

Knowledge of HIV structure and critical genomic elements. Ability to generate and refine large nucleotide alignments of diverse sequences

Primer and probe design (PCR, qPCR, NGS, etc.)

Extensive NGS knowledge and library prep experience (PacBio, Illumina, SMART-seq, etc)

Willingness to take up new skills and responsibilities

Ability to follow instructions

Capability of working independently in a teamwork environment

Have excellent interpersonal and communication skills

Competency with Microsoft Excel, Word, and PowerPoint, Geneious Prime