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CHA Hollywood Presbyterian Medical Center · 2 months ago

Research Associate II - Molecular and Cellular Biology

United States-California-Duarte

Full-time

Onsite

Entry, Mid Level

$33/hr - $51.15/hr

2+ years exp

CHA Hollywood Presbyterian Medical Center is at the forefront of groundbreaking research, particularly in the fight against cancer and diabetes. The Research Associate II position involves performing laboratory molecular and cellular biology techniques to understand pancreatic beta-cell development and diabetes pathogenesis.

Health CareHospitalMedical

Responsibilities

Perform a variety of routine and non-routine experiments utilizing a broad spectrum of molecular and cellular biology techniques to include but not limited to: mammalian cell culture including primary cultures and stable cell lines, iPSCs, DNA and RNA isolation, RNA and spatial sample preparation, sequencing library preparation, RT-PCRs, Western blot, immunohistochemistry, tissue collection and processing, islet physiology in vivo and in vitro, mouse genetics, confocal imaging and image analysis

Develop and optimize new laboratory methods and help refine existing methods, troubleshoot experimental procedures if experiment outcomes are unexpected or unsatisfactory

Accurately record experimental plans and work performed including results and methodology as well as analyze and compile data obtained

Lead the management of laboratory inventories, prepare stock solutions, handle and dispose biohazardous and non-biohazardous materials according to safety protocols when necessary

Train lab staff in specialized procedures, equipment and theoretical concepts behind unique tests

Contribute to the preparation of manuscripts and presentations

Qualification

Molecular biology techniquesCell cultureDNARNA isolationQRT-PCRWestern blotHistological analysisR PackagePythonTraining lab staffOrganizational skills

Required

Bachelor's degree in Biological Sciences or related field, with 2 years of post-degree research experience, or 1 year with a Master's degree

Experience in an academic or industrial laboratory setting

Familiarity with routine molecular and cellular biological techniques, such as DNA and RNA isolation, qRT-PCR, Western blot, cell culture, and other related techniques

Proficiency in MS Office applications, R Package or Python

Flexible and available to work on weekends when required to meet project deadlines or team needs