St. Jude Children's Research Hospital · 2 days ago
Cell Assay Lead/Senior Scientist - Blue Sky Kinases
Memphis, TN
Full-time
Onsite
Senior Level, Lead/Staff
$126K/yr - $238K/yr
7+ years expSt. Jude Children's Research Hospital is seeking an experienced cell biologist for the Cell Assay Lead/Senior Scientist position as part of the Blue Sky Initiative. The role involves conducting research on the human protein kinome, developing cell-based assays, and analyzing kinase-mediated signaling pathways to discover new biology and therapeutic opportunities.
BiotechnologyCharityHealth CareHealth DiagnosticsHospitalMedicalNon Profit
Responsibilities
Research kinase-mediated signaling pathways, kinase targets or kinase-dependent phosphorylation events, and cellular outcomes of such pathways for assigned kinase projects
Identify appropriate cellular assays and cells to perform structure-guided analysis of kinase signaling output and cellular phenotypes related to structure-guided perturbations in kinase regulation or function
Develop strategies and plans for implementing cell-based assays, including but not limited to evaluation of protein phosphorylation, protein interaction, protein localization, RNA abundance (qRT-PCR), protein ubiquitination, and protein abundance
Develop strategies and plans for implementing cell-based phenotypic assays, including but not limited to viability, apoptosis, autophagy, migration, or differentiation
Perform optimization and troubleshooting of cell-based assays
Perform statistical analysis using graphing software or R scripts
Interpret, analyze, and communicate cell-based assay results within the Cell-Based Assays team and with collaborators on specific kinase projects
Qualification
Required
Bachelor's degree in relevant scientific area is required
Experience in analysis of protein kinases and phosphorylation-dependent signaling events or pathways
Expertise in culturing a variety of types of cells, such as cancer cell lines, primary cells, mouse embryo fibroblasts, inducible human pluripotent cells, immune cells, neuronal cells, and muscle cells
Expertise in multiple methods for exogenous protein expression
Proficiency in presentation of experimental design and results using PowerPoint or similar software
Cloning, PCR, and qRT-PCR
Sterile technique
Cell transfection, transduction, or infection to exogenously express proteins of interest transiently or for cell line generation
Quantitative immunoassays, such as ELISA, AlphaLISA, and Mesoscale Discovery
Immunoprecipitation and Western blotting
Protein-protein interaction analysis, such as proximity ligation, FRET, or NanoBit assays
Flow cytometry and fluorescence-activated cell sorting (FACS)
Reporter gene assays
Phenotypic assays, such as viability, apoptosis, proliferation, migration, colony-forming, autophagy, endocytosis, secretion, or differentiation assays
Microscopy, such as immunofluorescence, live-cell imaging, subcellular localization, colocalization analysis
Cellular fractionation
Creative and results-driven with strong critical thinking and the ability to learn quickly
Hardworking, well-organized, flexible, and capable of multitasking efficiently in a fast-paced environment
Collaborative and efficient member of teams with diverse backgrounds and opinions
Ability to prioritize work and complete assignments promptly
Ten (10) years relevant work experience is required with a Bachelor's degree
Eight (8) years of relevant work experience is required with a Master's degree
Five (5) years of relevant work experience is required with a PhD
Twelve (12) years of relevant work experience is required with a Bachelor's degree for Lead Scientist
Ten (10) years of relevant experience is required with a Master's degree for Lead Scientist
Seven (7) years of relevant work experience is required with a PhD for Lead Scientist
Preferred
Experience with mouse models, preferred but not essential
Benefits
World-class facilities
State-of-the-art technologies
Extraordinary support
A collaborative, bench-to-bedside environment
Work-life balance with generous paid time-off and on-campus conveniences
Company
St. Jude Children's Research Hospital
St.
5001-10000 employees
H1B Sponsorship
St. Jude Children's Research Hospital has a track record of offering H1B sponsorships. Please note that this does not
guarantee sponsorship for this specific role. Below presents additional info for your
reference. (Data Powered by US Department of Labor)
Distribution of Different Job Fields Receiving Sponsorship
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Trends of Total Sponsorships
2025 (370)
2024 (316)
2023 (309)
2022 (255)
2021 (251)
2020 (201)
Funding
Current Stage
Late StageTotal Funding
$62.4MKey Investors
Kia MotorsKBR Charity Golf TournamentHyundai Hope On Wheels
2025-05-12Grant· $1.5M
2024-10-28Grant
2024-08-28Grant· $0.4M
Leadership Team
Judy A. Habib
Chair, Board of Directors
Recent News
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